Molecular Devices' microplate readers. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. . . molecular devices gmbh tel. Compare this item. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. In . The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Wavelength Selection. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. The reader adds up to three transfers of assay reagents from a 96- or 384. book Page 9 Wednesday, July 7, 2010 2:09 PM. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. 2 W x 44. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. #ID3-STD) • L-Tryptophan (Sigma cat. Or scan up to 384 wells at any time to determine the best assay conditions. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. This reader's dual monochromators for. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. 5 mm and 15. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Description. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). the appropriate well of the microplate. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. 76 L x 85. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Align the two slots on the bottle holder with the two pegs on the rail. 0 96 Well Plate. We would like to show you a description here but the site won’t allow us. Detergent Compatible Bradford assay. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 384 Awareness Technology ChroMate BioTek Synergy Mx. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Fluorescent DNA quantitation. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. D. 98 VMax R2 = 0. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Listing: #4772697. tools to expand the boundaries of their research. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. Molecular Devices has provided scientists with . Learn more about how absorbance is measured, and some key applications that utilize. #W50-100). flat-bottom: 50-60 µl. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. (FP) configured microplate readers. 003 fmol 0. Microplate reader assays requiring infrared (IR) or red-shifted. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. Table 1: Categories of heating element design. Reconstitution of. 0. SpectraMax 250 User. Built in near field communication (NFC). #L6876) Guanidine hydrochloride (Sigma cat. 6:30 . Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The FilterMax F3 can read 96-well and 384-well plates. explore more. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. While they play an important role in generating data from a wide range of. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Microplate readers can be either single-function instruments or “multimode. 100 fmol 1536-well (8 µL) 0. Microplate luminescent reader assay for ERK activity. fluorescence microplate reader from Molecular Devices. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. The system provides options for 20-, 40-,. Photometric and kinetic analysis mode. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. It includes both shaking and incubation functions, and is compatible with 96- well microplates. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. A control well was set up to verify. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. and luminescence microplate reader validation DATA SHEET. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Paradigm: Delta mP. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. In . We would like to show you a description here but the site won’t allow us. An example of dual-luciferase assay results. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. Standard SBS 96 and 384 well plates (128. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. (Figure 1). Condition: Used - Good. Keywords: fluorescence polarization, trf, fret, igg. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The Absorbance 96 is a uniquely designed microplate reader for assays in 96-well format. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. When the user applies a set temperature of 37°C, the top is heated to 37. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. CloneMatrix. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. Wide range of applications The VersaMax reader covers. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. All stand-alone instrument functions can be controlled using the software. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. 0 500 1000 1500 2000 2500 3000 3500 time (sec. Integrated stacker option. 39. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. SpectraMax 250 User. A preconfigured. Reader. Molecular Devices provides. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. 96-well: 0. Monochromator, tunable in 1-nm increments. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. #SpectraMax L Config) Methods. HPV Serology Laboratory Standard Operating Procedure . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. 6. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Reading a Microplate . Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. 96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-VolumeLearn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. Published Dec 16, 2020 | Updated Dec 16, 2020. Or scan up to 384 wells at any time to determine the best assay conditions. in a 96-well microplate. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Growth medium was then replaced with fatty acid uptake solution. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. #N207) LAL Reagent Water (Lonza cat. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. 76 L x 85. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. ©2010 molecular Devices, inc. SmartReader 96: Designed for optimal ELISA Testing. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 125 pM * Specifications listed are for europium 96-well: 0. Use black microplates. Optimized for fluorescence microplate readers. Integrated stacker option. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Step 1: Turn on your microplate reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 5. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 2 mm x 86 mm) Height: between 13. Read one sample or up to 384 in a. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. System setup and calibration are controlled from within SoftMax Pro Software. g. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. (FP) configured microplate readers. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Important things to consider when purchasing a multimode microplate reader. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. flexibility of monochromator-based systems. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. 2 OD at 200 nm. #SpectraMax L Config) Methods. $489. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Making it possible to perform kinetic analysis of solid. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. Molecular Devices i3x. System setup and. Use standard plate unless you see your type listed. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). (96, 384, or 1536 wells) and the "Select Specific". Quantitate proteins rapidly with a 5-minute, room-temperature incubation. NanoRacks added it to their line of commercial . 96-well solid bottom, clear polystyrene microplates (Greiner cat. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. Please refer to the Obsolete Microplate Reader Models for more information. 96-well-plate-scan-time-2-colors-1-site-well. The SpectraMax ABS. 05. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. In addition, the iD5 reader measures TRF and FP and. The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Other technologies such as imaging. Outer wells are aligned for proper viewing with FLIPR. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 48 W x 12. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Read the microplate Step 1: Place the microplate in the reader. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. BMG LABTECH. Expt#3 . The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. #G7294). ZERO BIAS. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. maximum-illumination-channels. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Ordering information FlexStation 3 Microplate Reader Part Number: FLEX3 • FlexStation 3 Base System Consumables 96-Well, FlexStation Pipet Tips (Black) Part Number: 9000-0911 • 200 µL capacity • Includes 3 vials each of FLIPR Calcium 6 and 6-QF dyes Reagents FLIPR® Calcium 6 Assay Evaluation Kit Part Number. Compare specifications and request a quote from leading suppliers. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. a white 96-well half-area microplate (Corning cat. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. Instrument settings can be saved as a protocol file and used repeatedly for reading differentMolecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. • 0. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Please refer to the Obsolete Microplate Reader Models for more information. Bioz Stars score: 86/100, based on 1 PubMed citations. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. AquaMax 4000 Microplate Washer (Molecular Devices Cat. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W (ABS Plus) • Option to run samples as low as 2 μL with the SpectraDrop™ Micro-Volume Microplate • Runs various assays within the Vis/UV-Vis range such asI tried Nunc Edge 2. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 030 fmol Fluorescence Polarization (FP). This . Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 96- or 384-well microplate compatibility. Compare plate readersThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Documentation:1. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Fluorescence Microplate Readers. 2 2601A ValitaTiter. Microplate Reader (Molecular Devices cat. The Perkin Elmer Wallac 1420. Shaking: Linear and Orbital. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. This can increase throughput and efficiency, thus saving time and space in the lab. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. or ValitaTiter Plus (Molecular Devices cat. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. Cells were loaded with 2 µM Fluo-4 for one hour. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 2 OD at 200 nm; this decreased to 0. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. #PLUS 384) Methods Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a black, clearbottom microplate. 03 pM 384-well: 0. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Average StDev (mP) 1. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. With various on-board features and capabilities, the ThermoMax is. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. We would like to show you a description here but the site won’t allow us. 50 racks/case for 384-well (16 channel pipettor head. 54: 1. 0. Comprehensive data analysis software. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Click on the crosshair and move it so that it is centered over the well image. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. ©2017 Molecular Devices, LLC . ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. The CellTiter-Glo assay from Promega uses luciferase. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. , Sunnyvale, CA 94089. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. While "plate reader" usually refers to the devices described. # 651101) the iD5 reader’s filter system. #M5e) Methods Microplate reader compatibility test. VANTAstar™ Flexible Multi-mode Microplate Reader. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. i3x. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Growth medium was then replaced with fatty acid uptake solution. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Temperature control. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. Detection modalities are shown in Table 1-1. Published Dec 16, 2020 | Updated Dec 16, 2020. 7°C (gradient heating design). Multimode Reader Comparison Chart. 1. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. white 96 well plate and 75. 96-well microplate; Immunoassay analyzer;VersaMax price. Formats available are strip washers, full plate washers and combination washer-dispensers. #ID3-STD) L-Tryptophan (Sigma cat. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. 0 OD at. 63. Page 154: Ordering Parts And Accessories. Find a new or used microplate reader and lab equipment for sale and auction at LabX. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. The VersaMax and SpectraMax 190 read 96-well microplates. Figure 2. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. New SpectraMax iD5 Multi-mode Microplate Reader. SpectraMax Microplate Readers. Reading Capabilities: cuvette or microplate. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate.